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KMID : 0353420210450030115
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Oral Biology Research
2021 Volume.45 No. 3 p.115 ~ p.118
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Comparison between the Rapid ID 32 Strep test system and 16S rRNA gene sequencing for the identification of α-hemolytic streptococci
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Lee Si-Young
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Abstract
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Oral viridans streptococcus is the most common bacterial species present in human dental plaque. The Rapid ID 32 Strep (bioMerieux, Marcy-l‘Etoile, France) test system allows for the identification of most streptococci within 4 hours. The aim of this study was to evaluate the reliability of this test in identifying isolates of α-hemolytic oral streptococci in human dental plaque and compare it with that of the 16S rRNA sequencing method. A total of eighty-five α-hemolytic streptococcal isolates were identified using 16S rRNA gene sequencing and Rapid ID 32 Strep. The Rapid ID 32 Strep method correctly identified 80% of the species and 87% of the streptococcal groups but failed to accurately identify five isolates (three S. cristatus, one S. australis, and one S. tigurinus).
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KEYWORD
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Identification, Sequencing, Streptococci
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